2.6. Chromatographic conditions
The HPLC analysis was performed with the prevailing carbohydrate
column (S5 lm, 250 mm 4.6 mm i.d) isocratically following
HPLC conditions: flow rate = 1.0 ll/min, data rate = 1 pps, run
time = 15 min, gain = 1, column heater temperature = 35 C, sample
temperature = 5 C, pressure = 50 psi, Nebulizer: heating (90%)
and injection volume = 10 ll. Acetonitrile:water mixture (70:30)
was used as the mobile phase.