and by 16S
rRNA sequencing. ITS regions represent highly conserved sequences in the areas encompassing the rRNA genes and relatively
stable regions located at the ends of the spacer. ITS-PCR showed a
very significant diversity with the detection of 7 distinct haplotypes
(Fig. 4). Each ITS-type was composed by 1 up to 5 reproducible
bands showing an apparent molecular weight (MW) ranging from
150 to about 950 bp. The most represented pattern was haplotype
H1, found in 3 isolates and composed by 2 reproducible bands of 950 bp and 750 bp. The other ITS types were found in two isolates.
Partial 16S rRNA gene sequencing was performed for 7 haplotypes
and was analyzed using BLAST algorithm (Table 2). Partial 16S rRNA
sequences together with their closest relatives were used for the
construction of neighbor-joining phylogenetic tree