2.10. Effect of ListShield™ on environmental L. monocytogenes
contamination in a smoked salmon production facility
The smoked salmon production facility, located in Scotland, was
thoroughly cleaned and disinfected one day before the study was
initiated. On the day of the experiment, forty smoked salmon fillets
were obtained directly from the production line and studies were
performed in a laboratory located within the facility. Twenty of the
salmon fillets were experimentally contaminated with 2 103 CFU
of L. monocytogenes strain Lm376, and ten of these were also treated
with ListShield™ at an application rate of 1.5 106 PFU/g. The
remaining twenty fillets were not experimentally contaminated
and ten of these were treated with ListShield™ at an application
rate of 1.5 106 PFU/g and the remainder were treated with an
equal volume of sterile water. The smoked salmon fillets were then
individually sealed in bags and stored at 2e6 C for 24 ± 2 h.
Following cold storage, a 25 g sample of each fillet was suspended
in 225 mL of BLEB, stomached for approximately 30 s, and enriched
at 30 C for 48 h. To determine if the samples were positive for the
presence of L. monocytogenes, an aliquot was plated on Oxford agar
and the plates were incubated for 48 h at 30 C.
2.10. Effect of ListShield™ on environmental L. monocytogenescontamination in a smoked salmon production facilityThe smoked salmon production facility, located in Scotland, wasthoroughly cleaned and disinfected one day before the study wasinitiated. On the day of the experiment, forty smoked salmon filletswere obtained directly from the production line and studies wereperformed in a laboratory located within the facility. Twenty of thesalmon fillets were experimentally contaminated with 2 103 CFUof L. monocytogenes strain Lm376, and ten of these were also treatedwith ListShield™ at an application rate of 1.5 106 PFU/g. Theremaining twenty fillets were not experimentally contaminatedand ten of these were treated with ListShield™ at an applicationrate of 1.5 106 PFU/g and the remainder were treated with anequal volume of sterile water. The smoked salmon fillets were thenindividually sealed in bags and stored at 2e6 C for 24 ± 2 h.Following cold storage, a 25 g sample of each fillet was suspendedin 225 mL of BLEB, stomached for approximately 30 s, and enrichedat 30 C for 48 h. To determine if the samples were positive for thepresence of L. monocytogenes, an aliquot was plated on Oxford agarand the plates were incubated for 48 h at 30 C.
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