(d)E. coli isolate carrying the histidine6-greenfluorescent
protein plasmid (pH6GFPuv).
3.Distilled water.
4.0.5 McFarland standard no. 1.
5.NucleoSpin® Plasmid kit: Buffer, NucleoSpin column, collection tube.
6.Hot plate.
7.Spectrophotometer.
8.General lab materials and equipments: Microcentrifuge tubes,
pipette tips, pipettes, centrifuge, and disposable gloves.