Number of studies showed an application of fungal laccase in phenolic compound removal.Precipitation of phenolic compounds as aggregates on reaction with phenol oxidases is a well-established phenomenon. Formation of these polymerization products was an indication of typical laccase activity. In the present study no such aggregate formation was observed . This showed that the removal of phenolic compounds by the action of PPO enzyme. The time course of gallic acid degradation by PPO enzyme was shown in Table 4.