2.3. Free Radical Scavenging Assays
2.3.1. Total Antioxidant Capacity Assay
Aliquots of suitable working solutions (1 - 10 mg/ml) of
the samples were mixed with 1 ml of the reagent solution
(0.6 M sulphuric acid, 28 mM sodium phosphate and 4
mM ammonium molybdate) and incubated at 95˚C for 90
min [8]. The tubes were cooled to room temperature and
the absorbance was measured at 695 nm against a blank.
Ascorbic acid was used as a standard. Total antioxidant
capacity was expressed as equivalents of ascorbic acid
[9].