Tissue blocks of 1 to 2 mm are prepared for processing with appropriate
geometry to assure proper orientation. Alternative is to cut
60- to 100-micron sections on vibratome (allowing 2 to 4 mm of
block thickness and up to 1 × 1 cm block face) which facilitates
visual control of dissection and processing. Such sections can be
alternated with 15- to 35-micron sections for light microscopy and
immunocytochemistry.