Biopsies of the wounded areas were performed for histological evaluation tissue on the days 7th and 14th, transferred inside the container containing10% buffered formalin, and were sent to the laboratory. These samples were prepared as sections with 5 micrometer diameters and after hematoxylin and eosin (H & E)staining, studied with an optical microscope (4Nikon, Japan) with magnifications of, 10 and 40