then rinsed under running sterile water for 1 min and air-dried. Each fruit was wounded with a 5 mm diameter hole-puncher and injected with 5 mm diameter mycelia plugs of C. gloeosporioides or 50 lL C. gloeosporioides conidial suspension (105 conidia mL_1) in the wound area. To study the antagonistic activity of volatiles on mycelia growth or conidial germination, ten-inoculated fruits were
arranged in a 6.5 L container as one replicate of each antagonist strain. Then, 20 lL suspensions of the presumptive bacterial antagonists (1 _ 106 cfu mL_1) were inoculated separately on NA for 24 h at 37 _C. Also, six cultures were placed in the container with no direct contact with the mangos. For the control groups, sterile water of the same volume replaced the antagonist cultures. After 7 days at 25 _C, the diameters of decayed spots were measured and the lesion area was calculated to contrast treatment effects and antagonistic activity. Three replications of each treatment were performed and the experiment was repeated three times.