Common health problem in Chinese University is Self-medication with antibiotics. Especially the use of non-prescription antibiotics. From the problems of Self-medication with antibiotics. The research about Self-medication with antibiotics and risk factors among Chinese university students by online questionnaire. The result an online survey at Jiangsu university (JSU). More than half of population have been used Self-medication with antibiotics and changed antibiotic dosage and antibiotic during course. Self-medication with antibiotics as a risk factors in older age and prior knowledge especially in female is exclusive to risk factors Therefore, should have strict regulations and recommended about Self-medication with antibiotics.