Nitrocellulose membrane
was cut into sections (2.5 cm × 26 cm). Test line was coated with
TSWV-IgG conjugate, which was applied to each membrane in
150 μg/ml TSWV using TLC conjugate sampler (Sambrook et al.,
1989). The distance between the test line and control line was
6 mm. The test strips were dried at 37°C for 30 min.