with two or more of the following: headache,
retro-orbital pain, myalgia, arthralgia, rash, haemorrhagic
manifestations, or leukopenia; and a confirmed case of
dengue was also associated with one or more of the following:
supportive serology (reciprocal haemoagglutination–inhibition
antibody titre, comparable immunoglobulin G (IgG) enzymelinked immunosorbent assay (ELISA) titre, or positive
monoclonal IgM antibody capture (MAC-ELISA) test in a
serum specimen from the late acute or convalescent phase.9
Clinically suspected dengue cases were tested with a rapid
test kit such as NS1 antigen (nonstructural antigen 1 rapid
diagnostic) test kit. Since a dengue referral laboratory was not
available at the outbreak district, blood samples were also sent
to Midnapore Medical College Hospital for MAC-ELISA test,
for confirmation of dengue virus. Use of the NS1 ELISA and
IgM ELISA (MAC ELISA) tests was recommended by the
West Bengal Government for confirmation of dengue at public
health laboratories.