All measurements were performed using a commercially available kit (Randox, N Ireland). Samples were analyzed
spectrophotometrically (Spectronic Genesis 10 Bio, Thermo Scientific, USA) at 340 nm at baseline and 1, 2, and 3 min of incubation. Creatine kinase activity was determined by substituting the change in absorbance between baseline and 3 min timepoint (ΔA 340 nm/min) into the following formula: Creatine kinase activity u/l = 4127 × ΔA 340nm/min