The seven bacteria studied belong to the Laboratory of
Microorganisms Genetics Collection (LABGEM) of the
Federal University of Parana, and are deposited at the
Diazotrophic and Plant Growth Promoting Bacteria
Culture Collection of Embrapa Soja (WDCC Collection
# 1054), with the record numbers from CNPSo 2475 to
CNPSo 248.GeneBank accession numbers for the 16S
rRNA go from JQ821359 to JQ821365. These bacteria
were isolated from corn roots samples by Ikeda et al.
(2013). For the isolation, corn roots from field trial
conducted at Semilia Genetics and Breeding LTDA,
located in the southern Brazilian region of Campo Largo-
PR, were surface-sterilized (Petrini, 1986) and aseptically
cut, then transferred to Petri dish plates containing
solid culture media. Several controls confirmed that the
sterilization procedure was effective.