Real-time turbidity measurement by a cost-effective
photometer with incubation function has enabled the
kinetic analysis of the LAMP reaction without the need
for any detection reagents such as a fl uorescence
intercalator. This is because the LAMP reaction produces
insoluble magnesium pyrophosphate in the course of the
amplifi cation reaction. It has been reported that the realtime
turbidity analysis of LAMP makes it possible to
quantify the initial amount of template DNA in the
samples.15 Furthermore, a LAMP positive reaction can be
easily detected by visual endpoint judgment of turbidity
(Fig. 2A).16 The development of such a simple detection
method as well as an inexpensive real-time turbidimeter
has contributed to the acceptance of the LAMP
amplifi cation method.