1. Take required number of plastic petri dishes. 2. Disinfect the surface of the plates. 3. Place the seeds (25 seeds) in a petri dish on three wet blotter papers in three rows circularly (16+8+1). 4. Place the petri dishes for incubation at 20º C in suitable condition. 5. Examine the fungi on seed under stereomicroscope. 6. Observe growth characters and identify the fungi. 7. Count the number of infected seeds and calculated percent seed infection.