Water sampling and analysis of physico-chemical parameters
Four seasonal collections were made from January 2011 to
December 2011. Samples were collected from each station (four
seasons×three stations×six replicates). Rainfall data were collected
from the metrological department office at CAS in Marine Biology,
Annamalai University. Muthupettai for station I and Parangipettai for
station II and Pamban for station III. Water samples were collected
in pre-cleaned polypropylene containers, just below the water surface
separately from the sampling sites. After collection, all the samples were
cooled and then brought to the laboratory in an insulated thermocool
box. Soon after returning to the laboratory, the water samples were
filtered through a Whatman GF/C filter paper for nutrient analysis.
Water temperature was measured using a mercury centigrade
thermometer with 0.5°C accuracy. The pH of samples was measured by using a calibrated pH pen (Phep; Hanna instruments Mauritius
Ltd., Portugal) with an accuracy of ± 0.1. The pH in the solution
was measured using a pH meter, calibrated with standard buffer
solution prior to use. The salinity of samples was measured by using
a hand refractometer (Atago, Japan). Water samples were transferred
carefully to BOD bottles. The modified Winkler’s method described by
Strickland JDH and Parsons TR [13] was adopted for the estimation of
dissolved oxygen fixed. The nitrate, nitrite, inorganic phosphate, and
reactive silicate content of water samples were analyzed by the method
of Strickland JDH
Water sampling and analysis of physico-chemical parametersFour seasonal collections were made from January 2011 toDecember 2011. Samples were collected from each station (fourseasons×three stations×six replicates). Rainfall data were collectedfrom the metrological department office at CAS in Marine Biology,Annamalai University. Muthupettai for station I and Parangipettai forstation II and Pamban for station III. Water samples were collectedin pre-cleaned polypropylene containers, just below the water surfaceseparately from the sampling sites. After collection, all the samples werecooled and then brought to the laboratory in an insulated thermocoolbox. Soon after returning to the laboratory, the water samples werefiltered through a Whatman GF/C filter paper for nutrient analysis.Water temperature was measured using a mercury centigradethermometer with 0.5°C accuracy. The pH of samples was measured by using a calibrated pH pen (Phep; Hanna instruments MauritiusLtd., Portugal) with an accuracy of ± 0.1. The pH in the solutionwas measured using a pH meter, calibrated with standard buffersolution prior to use. The salinity of samples was measured by usinga hand refractometer (Atago, Japan). Water samples were transferredcarefully to BOD bottles. The modified Winkler’s method described byStrickland JDH and Parsons TR [13] was adopted for the estimation ofdissolved oxygen fixed. The nitrate, nitrite, inorganic phosphate, andreactive silicate content of water samples were analyzed by the methodof Strickland JDH
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