small pieces (3–5 cm length), ground with a Pulverisette 14 variable-speed
rotor mill (Fritsch, Germany) and sieved through a
106 lm screen prior to analysis. Samples were gelatinized with a
heat-stable a-amylase (pH 6, 100 C, 30 min) and then enzymatically
digested sequentially with protease (pH 7.5, 60 C, 30 min)
and amyloglucosidase (pH 4.5, 60 C, 30 min) to remove protein
and starch. TDF was precipitated with ethanol, and after washing
and drying, the residue was weighed.
T
small pieces (3–5 cm length), ground with a Pulverisette 14 variable-speedrotor mill (Fritsch, Germany) and sieved through a106 lm screen prior to analysis. Samples were gelatinized with aheat-stable a-amylase (pH 6, 100 C, 30 min) and then enzymaticallydigested sequentially with protease (pH 7.5, 60 C, 30 min)and amyloglucosidase (pH 4.5, 60 C, 30 min) to remove proteinand starch. TDF was precipitated with ethanol, and after washingand drying, the residue was weighed.T
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