1 0.05 % Polyacrylamide. Weigh 0.25 g polyacrylamide to 450 ml of double RO water and stir overnight with a magnetic stirrer. Make up to 500 ml (Do not try to add the double RO water to the polyacrylamide.)
4.2 1N Sodium hydroxide for pH adjustment. Weigh 20 g of sodium hydroxide, dissolve and dilute with double RO water to 500 ml.
4.3 0.5 M sodium bicarbonate, pH 8.5 with polyacrylamide (extracting solution). Weigh 168 g of sodium bicarbonate and place in a 4L Erlenmeyer flask. Dissolve in about 3,900 ml double RO water. Add 20 ml of 0.05% polyacrylamide. Adjust pH to 8.50with 1N sodium hydroxide (approximately 60-80ml). Add enough double RO water to make to 4 L if necessary.