Research on sea slugs production has steadily increased in the last decades as a result of their use as model
organisms for biomedical studies, bioprospecting for new marine drugs and their growing demand for
academic research and the marine aquarium trade. However, standardized methods for culturing sea slugs
are still limited to a reduced number of species. The main bottlenecks impairing sea slugs aquaculture are
the lack of knowledge on suitable larval diets and settlement cues that can inducemetamorphosis in competent
larvae. Additionally, the stenophagous feeding regime displayed by several species requires the collection and/or
culture of their prey, which commonly impairs large-scale production. Nevertheless, significant breakthroughs
have been achieved in recent years through the development of innovative culture techniques. The present review
summarizes the major issues impairing the culture of sea slugs and presents relevant biological and ecological
data that can assist on the development of suitable culture protocols. Information on themost suitable husbandry,
larviculture and grow-out techniques are critically discussed, with emphasis to their application on some of the
most relevant groups of sea slugs from an academic and commercial point of view: sea hares (Aplysia spp.),
nudibranchs (e.g., the marine ornamental species Aeolidiella stephanieae) and the “solar powered” sacoglossan
(e.g., Elysia spp.).