Bacterial contamination can cause a major loss of micropropagated plants. Contamination in plant tissue culture can originate from explants,laboratory environments, operators, or ineffective aseptic techniques. Inaddition, plant parts, stage of plants, plant species and sources of plants are also the causes of the bacterial contamination in the plant tissue. Temporary immersion bioreactor system is a micropropagation procedure that empolys the use of automation technique to control rapid multiplication of plant cultures and applied for plant propagation in industrial level. The characterization of bacteria and the anylyzing processes may provide important information about sources of contamination, how to prevent or reduce bacterial contamination. Therefore, the objectives of this study were to carry out the sources of bacterial contamination and to find the method for bacterial contamination control in TIB cultivation system.