The DNA-invertase was used to catalyze the hydrolysis of sucrose into glucose for PGM readout.the enrichment effect of Streptavidin-MNBs and the million turnovers of sucrose hydrolysis into glucose. In addition, the method exhibited excellent sequence selectivity, being able to there was almost no effect of biological complex to the detection performance, which suggested this method can be success-fully applied to DNA detection in real biological samples.