Based on this property of ABA, a series of
mini-preparing columns/cartridges were screened for the performance
to adsorb ABA at pH 3.0 and to desorb ABA at pH 9.0. The
Supelclean ENVI-Chrom P column showed good performance on
ABA adsorption in acidic conditions and desorption in alkali conditions.
As shown in Fig. 3b, in acidic condition (pH 3.0), standard
ABA was loaded on to the column and the column was washed or
eluted with different concentrations of acidic ethanol. ABA could
not be eluted from the column with up to 40% ethanol. This result