More than half of the lactic acid in all cultures except pH 8 at 26 and 28◦C was consumed. Fig. 2 also shows that acetic acid was more easily metabolized than lactic acid by Acetobacter xylinus 23769. The glycolic acid and formic acid concentration increased after cultivation. Amounts of acetic acid, glycolic acid, lactic acid and formic acid increased slightly during autoclaving compared to Hot water extract before autoclaving which can be attributed to sugar degradation during thermal treatment. Guo et al. (2013)observed similar phenomena. The effect of pH and temperature on the concentrations of four monosaccharides and five inhibitors were negligible.