Yeast strain and culture growth conditions:
Saccharomyces cerevisiae (KF233529) has been
isolated from honey and standardized in the
Department of Agricultural Microbiology was used for
this study. The yeast cells were grown in Yeast
peptone dextrose agar (YPD), containing glucose 20g,
peptone 10g, yeast extract 5g and agar 20g per litre.
The culture was routinely maintained at 4ºC on slants.
Before use, the culture was transferred to YPD broth
and incubated for 24 h at 27º C.