Oryzanol content of blended oil was determined by a spectrophotometric
method (Gopala et al., 2006) by dissolving 0.01 ml
of the sample in 10 ml of hexane and reading the absorbance at
314 nm in a 1-cm cell (Systronics UV–VIS-108 spectrophotometer,
Bangalore, India). The oryzanol content was calculated by using the
formula: