Saposin-like protein 2 (SAP-2) is a protein that adult of Fasciola spp. use to lyse plasma membrane of red blood cells, so that their contents can be digested by proteases for the parasites nutrients.Thus SAP-2is a plausible target for vaccination against these parasites. Recombinant Fasciola gigantica saposin-likeprotein 2 (rFgSAP-2) was expressed in Escherichia coli BL21 (DE3).