The subset of 19 markers selected from the total of 98 markers provided the resulting groups corresponding to the Indica and Japonica subspecies, and correlated quite
well with their ecologies and their known information on their development. Previous study showed that genetic diversity was also determined in genus Orysza using
11 ISSR markers selected from 30 ISSR markers (Joshi et al. 2000). Small numbers of markers can be used to estimate genetic diversity as shown in the earlier study when a subset of 30 markers provided the same results as using all 111 markers with the same genetic distance matrices and dendrograms (Ni et al. 2002). Similarly, Ali et al. (2011) also showed that a subset of 36 SSR markers gave nearly the same results as using 169 SSR markers for population structure analysis.