.Antioxidant activity of liquid smoke
The measurement of the liquid smoke's antioxidant activity has been
done using the DPPH assay (2.2′-diphenylpicrylhydrazyl) according to
methodology described by Miranda and Fraga (2006) with some modifi-
cations. It consists of in incubation for 30 min, 500 μL of 0.1 mM DPPH
(2.2-diphenyl-1-picryl hydrazyl) in ethanol with 500 μL of solutions containing
growing concentrations of liquid smoke in ethanol. The control
solution was obtained replacing 500 μL of liquid smoke for 500 μL of
ethanol, without DPPH. Absorbance measurements were then taken at
517 nm. The measurement of antioxidant activity on commercial
additives BHA (butylhydroxyanisol) and BHT (butylhydroxytoluene)