The carbohydrate oxidase (COXMn) from Microdochium nivale may well have desired functionalities as a
dough and bread improver, similarly to Aspergillus niger glucose oxidase (GOX). COXMn catalyses the
oxidation of various monosaccharides as well as maltooligosaccharides for which the best activity is
obtained towards the maltooligosaccharides of polymerisation degrees 3 and 4. For the same activity
towards glucose under air saturation, we show that COXMn exhibits a similar efficiency towards maltose
as GOX towards glucose whatever the oxygen supply. Assays with COXMn show that no competition
exists between carbohydrates naturally present in the wheat flour. We show that reaction products
(D-glucono-d-lactone and hydrogen peroxide) and the wheat flour dough component, ferulic acid, have
no noticeable specific effect on the COXMn activity. The demonstrated differences in kinetics between
COXMn and GOX allow predicting of differences in the functional behaviours of those enzymes during
wheat flour dough formation.