Methanol and ethanol and/or aqueous methanol and ethanol are also good solvents for the extraction of bio-active principlesfrom CS.
These alcoholic extracts are rich in very potent bio-actives like nucleosides, polysaccharides, proteins.
Etha-nolic extract shows strong anti-oxidant activity (Yamaguchi
et al., 2000b), preserve b-cell function and offer renoprotection
(Kan et al., 2012).
Methanolic extract obtained from culture liquid of CS also showed cytotoxic effect against cancer cell lines (Jia et al., 2009).
More about pharmacological effects of these extracts as such or purified fractions of extracts are discussed in later section.