2.5 lmmunity analysis
Prepartion of hemolymph samples followed the method of Smith and Soderhall
One hundred มิวแอล hemolymph was withdrawn from the ventral sinus cavity of each living shrimp with 1-ml sterile syringe (25 gauge) containing 200 มิวแอล of anticoagulant solution (100 mM EDTA•Na2, 450 mM NaCl,10 mM KCl,10 mM HEPES, pH 7.3 and the osmolarity was adjusted with glucocse to 850 mosm/kg).