After the flask cooled down, the fungal mycelium
on the culture plates was cut in disks, 5 mm in diameter, and
placed in the center of the broth-cultured flask. The cotton
wool stoppers were then wrapped with aluminum foil, sealed
with parafilm, and incubated at 30 ± 2 C (12-h-darkness/
12-h-light cycle) on a rotary shaker for 14 days at 120 rpm
for the culturing of A. niger.