Three biopsies for realtime
PCR were taken with endoscopic fenestrated alligator
tooth biopsy forceps (Olympus FB-36k-1;
Olympus, Vienna, Austria) and snap frozen in liquid nitrogen
and stored at 80C until use, whereas another
four biopsies were fixed in 4% formaldehyde for histological
examination. After recovery from anesthesia, the
foals were returned to their dams. One foal from group
COL died 24 hours after anesthesia because of respiratory
problems.