The susceptibility of M. pachydermatis strains to antifungal
compounds was verified using the reference CLSI
M27-A2 with some modifications. In particular, Sabouraud
Dextrose Broth medium (Liofilchem Diagnostici1, Roseto
degli Abruzzi, Italy) with 1% of tween 80 (Sigma Co, Milano,
Italy) was used instead of RPMI 1640 medium which has
been shown to be unsuitable for the growth of M.
pachydermatis since it lacks the required lipid supplements
(Rinco´n et al., 2006)