4. ConclusionWith this study we found that the distribution of chitosan ontothe chitosan-coated paper is uniform and that this macromoleculedoes not have a preferential way to cover the surface of the paper.Chitosan penetration into the sheets occurs progressively with thefirst layers and after a supplementary coating onto the paper sheetis observed. Both reflectance and luminescence, showed a satura-tion of the FITC–CH-coated paper after the three layers. The exper-imental approach presented here to assess chitosan distribution onchitosan-coated paper may be certainly extrapolated to the studyof other paper-coating agents.