For inoculation purposes, it was cultured for 10 days at 22 °C on Potato Dextrose Agar (PDA) (Pronadisa, Madrid, Spain). The inoculum suspension was prepared by adding 10 mL of sterile purified water on the surface of a PDA plate where F. circinatum has grown for about 10 days at 22 °C, scraping with a spatula, and then filtering with glass wool washed