increase on ethanol production until 87.94 g/L. The ethanol produced by New Aule Baker’s Yeast under aerated
culture were significantly different or tend to be higher compare to its non-aerated culture, with ethanol produced at
120.92 g/L. Based on the explanation above, it showed that aeration affect the ethanol concentration produced by
both yeast indicated an increase in ethanol production. Correlated with Alfenore et al. (2004), reported that aeration
0.2 vvm during fed-batch fermentation could increase ethanol production from 128.1 g/L (non-aeration) to 143.8 g
g/L.
The different in ethanol concentration produced by both yeast was hypothesized due to its ability by both yeast to
adapt under such fermentation condition. The New Aule Alcohol Yeast tend to need longer time (48 hours of
incubation time) to produce maximum ethanol concentration under aerated culture, meanwhile New Aule Baker’s
Yeast only need the exact same incubation time (36 hours) to produce maximum ethanol concentration under
aerated culture. It was hypothesized that this condition could happen because of several factors, such as temperature,
pH, etc. New Aule Alcohol Yeast is popular for its thermal tolerance (best temperature to be 38-40o
C) and acid
tolerance (pH 2.5), so due to the uncontrolled temperature and pH during incubation time, this yeast couldn’t reach
maximum ethanol production, indicating further optimum fermentation condition need to be studied for this yeast.
The decrease in ethanol concentration occurred at the end of incubation time of both yeast. It was hypothesized,
both yeast were in a death phase, so that it couldn’t convert sugars to ethanol optimally. Throughout fermentation,
yeast was not only produce alcohol, but also by products. Mukhtar et al. (2010) stated that during alcoholic
fermentation, Saccharomyces cerevisiae was not only producing alcohol, but also by product such as organic acids
and acetaldehyde which can decrease the alcohol content. The alcohol accumulated in sample interacted with
organic acids and formed ester compounds and make the alcohol content of sample decreased.
increase on ethanol production until 87.94 g/L. The ethanol produced by New Aule Baker’s Yeast under aeratedculture were significantly different or tend to be higher compare to its non-aerated culture, with ethanol produced at120.92 g/L. Based on the explanation above, it showed that aeration affect the ethanol concentration produced byboth yeast indicated an increase in ethanol production. Correlated with Alfenore et al. (2004), reported that aeration0.2 vvm during fed-batch fermentation could increase ethanol production from 128.1 g/L (non-aeration) to 143.8 gg/L.The different in ethanol concentration produced by both yeast was hypothesized due to its ability by both yeast toadapt under such fermentation condition. The New Aule Alcohol Yeast tend to need longer time (48 hours ofincubation time) to produce maximum ethanol concentration under aerated culture, meanwhile New Aule Baker’sYeast only need the exact same incubation time (36 hours) to produce maximum ethanol concentration underaerated culture. It was hypothesized that this condition could happen because of several factors, such as temperature,pH, etc. New Aule Alcohol Yeast is popular for its thermal tolerance (best temperature to be 38-40oC) and acidtolerance (pH 2.5), so due to the uncontrolled temperature and pH during incubation time, this yeast couldn’t reachmaximum ethanol production, indicating further optimum fermentation condition need to be studied for this yeast.เอทานอลความเข้มข้นที่ลดลงเกิดขึ้นเมื่อสิ้นสุดเวลาการบ่มของยีสต์ทั้งสอง คือการตั้งสมมติฐานยีสต์ทั้งสองอยู่ในระยะตาย ดังนั้นมันไม่สามารถแปลงน้ำตาลเป็นเอทานอลอย่างเหมาะสม ตลอดการหมักยีสต์ไม่เพียงผลิตแอลกอฮอล์ แต่ยังผลิตภัณฑ์ Mukhtar et al. (2010) ระบุไว้ที่ระหว่างแอลกอฮอล์หมัก Saccharomyces cerevisiae คือไม่เพียงการผลิตแอลกอฮอล์ แต่ยังตามผลิตภัณฑ์เช่นกรดอินทรีย์และ acetaldehyde ซึ่งสามารถลดการแอลกอฮอล์ แอลกอฮอล์ที่สะสมในตัวอย่างติดต่อกรดอินทรีย์ และสารเอสเตอร์ที่เกิดขึ้น และทำให้แอลกอฮอล์ของตัวอย่างลดลง
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