A supplementation of 2.0 mg/l BAP and 0.5 mg/l NAA
gave the best result to stimulate a successful production
of multiple shoots of B. rortunda with 90% of the inoculated
explants inducing multiple shoots. Clonal propagation
and germplasm conservation of B. rotunda was
attractive because the propagated material was shown to
possess high genetic stability (Rout et al., 1998). In vitro
plantlets can be an important source of disease-free
planting material, ideally suited for germplasm exchange,
transportation and conservation.