Fig. 3 TLC analysis of lipopeptides secreted by Bs-916 and the mutant strains. Lane 1, Bs-M49; lane 2, standard surfactin; lane 3, Bs-916; and lane 4, Bs-H74. Samples (1 μl) were spotted on a silica plate, chloroforml/methanol/water (65:25:4) as the mobile phase