Preparation of meat hydrolysate The meat (chicken, pork or beef), which was ScienceAsia 26 (2000) 221 free of fat, was cut into small pieces and boiled for 10 min.
The cooked meat was ground and water was added to obtain 25% (w/w) of solid.
Papain was then added to the ground meat slurry at 0.5% (w/w) of the dried meat followed by incubation at 60°C for
12 h.
The enzyme was then deactivated at 90°C for 15 min.31,32 The meat hydrolysate slurry was then clarified by centrifugation at 10500 rpm for 15 min.
The clear supernatant was transferred to a separatory funnel and any surface oil removed.
The resulting solution was called meat hydrolysate.