Test compounds were applied in a single application as a soil
drench (500 mL of solution per pot) or as a foliar spray (100 mL of
solution per plant) using an airbrush (Crown Spra-Tool, Aervoe
Industries, Inc.). The timing of treatments and their rates were
chosen based in previous reports (Francis et al., 2009; Llorens et al.,
2013) of effective dosages and timing of applications. In brief:
acibenzolar-S-methyl (ASM; Actigard® 50WG, Syngenta Crop Protection)
used as a positive control was applied as a foliar spray at
1 mM 4 days before inoculation or as a soil drench applied 7 days
before inoculation; Hx was applied as a foliar spray at 1 mM or
3mMone day before inoculation or as a soil drench at 1mM4 days
before inoculation. In all the experiments, nontreated and inoculated
plants were included as nontreated checks (NTC). Inoculation
of all plants was made on the same day.
Test compounds were applied in a single application as a soildrench (500 mL of solution per pot) or as a foliar spray (100 mL ofsolution per plant) using an airbrush (Crown Spra-Tool, AervoeIndustries, Inc.). The timing of treatments and their rates werechosen based in previous reports (Francis et al., 2009; Llorens et al.,2013) of effective dosages and timing of applications. In brief:acibenzolar-S-methyl (ASM; Actigard® 50WG, Syngenta Crop Protection)used as a positive control was applied as a foliar spray at1 mM 4 days before inoculation or as a soil drench applied 7 daysbefore inoculation; Hx was applied as a foliar spray at 1 mM or3mMone day before inoculation or as a soil drench at 1mM4 daysbefore inoculation. In all the experiments, nontreated and inoculatedplants were included as nontreated checks (NTC). Inoculationof all plants was made on the same day.
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