Fusarium oxysporum is economically significant disease on tomato to causes Fusarium Wilt plant werecollected from North of Iraq, Kirkuk city were obtained from xylem tissues showing typical. Sections of vasculartissue were excised and placed aseptically on PDA medium. Plates were incubated at 22 c under 12 h photoperiodicuntil the development of fungal colonies. The Identification of Fusarium isolates was made on basis of theirmorphological characters. From the colony edge, monosporic cultures were generated, until a pure colony wasobtained, which served as base for the fungi multiplication .One F.oxysporum F.oxysporum monoconidial isolatewas obtained and maintained on PDA media at 22 c and used to antagonism study.