The work was carried out in the Laboratory of Plant Tissue Culture at the Biology Department, in the Sector of Plant Physiology at the Federal University of Lavras (UFLA), Lavras, Minas Gerais, for three months in 2009. Ripe fruits from commercial planting were used at Monteiro Farm in the county of Messias in Alagoas. The fruits were immersed in sodium hypochlorite solution of 0.2% for 30 minutes, and after that, washed with running water for 1 minute.