Konjac oligo-glucomannan (KOG) was prepared by degradation of konjac glucomannan (KG) using -mannanase.
The hydrolysis process was monitored by the viscosity of the enzymatic hydrolysates.
Factorsaffecting the enzymatic hydrolysis of KG were investigated, and the optimum hydrolysis conditions wereas follows: time 2 h; temperature 50◦C; pH 6.0; and enzymatic concentration 150 U/g.
Under these opti-mized conditions, minimum viscosity (31.9 mPa·s) of the hydrolasate was obtained.
The average degreeof polymerization (DP) of the resulting KOG was approximately equal to 5.2. The results of Fourier trans-form infrared (FTIR) spectra of KG and KOG indicated that KG was successfully degraded.
In addition,their antioxidant activities were evaluated by determination of hydroxyl radical (•OH) and 1,1-diphenyl-2-picrylhrazyl radical (•DPPH) scavenging activity, and determination of reducing power.
The resultsshowed that KOG exhibited significant antioxidant activities. Taken together, this study suggested thatKOG could potentially be used as a natural antioxidant.
Konjac oligo-glucomannan (KOG) was prepared by degradation of konjac glucomannan (KG) using -mannanase.The hydrolysis process was monitored by the viscosity of the enzymatic hydrolysates. Factorsaffecting the enzymatic hydrolysis of KG were investigated, and the optimum hydrolysis conditions wereas follows: time 2 h; temperature 50◦C; pH 6.0; and enzymatic concentration 150 U/g. Under these opti-mized conditions, minimum viscosity (31.9 mPa·s) of the hydrolasate was obtained. The average degreeof polymerization (DP) of the resulting KOG was approximately equal to 5.2. The results of Fourier trans-form infrared (FTIR) spectra of KG and KOG indicated that KG was successfully degraded. In addition,their antioxidant activities were evaluated by determination of hydroxyl radical (•OH) and 1,1-diphenyl-2-picrylhrazyl radical (•DPPH) scavenging activity, and determination of reducing power. The resultsshowed that KOG exhibited significant antioxidant activities. Taken together, this study suggested thatKOG could potentially be used as a natural antioxidant.
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