In searching for new antibiotics, relatively simple and rapid methods have been developed for screening microorganisms for antibiotic producing ability. Soil sample are commonly employed in the antibiotic producing organism. Bacillus species are ubiquitous in nature. Antibiotic isolated may be bactericidal or bacteriostatic in nature. Production of antibiotic by microorganisms from soil is affected by many factors including nitrogen and carbon source. Temperature variation also affects the synthesis of antibiotic. Antibiotic is synthesis during the idophase stage of the growth and there is no correlation between the bacteria sporulation and antibiotic production [9]. The soil bacteria isolated shows antibiotic activity under normal growth condition and was found inhibiting some gram positive and some gram negative organism both Bacillus lentus and Bacillus alvei shows antibacterial activity agains Staphylococcus aureus. Bacillus pumillus only show slight zone of inhibition on proteus spp while it is inactive against others. Micrococcus roseus was also proved to have antibacterial activity only against Shigella spp. Enterobacter aerogene and Bacillus alvei shows clear zone of inhibition against Pseudomonas spp but the former is higher than the later. Escherichia coli proved to be resistance to all the isolates that is there is no clear zone of inhibition observed against the bacteria