To study the effectiveness of protection for the green-colour surface, five water-borne copper-based preservatives, including ammoniacal copper quaternary compound-Type B (ACQ-B), copper azole-Type A (CBA-A), tanalith CY (TCY), copper azole-type B (CA-B) and micronized copper quaternary (MCQ), were used in the experiment (Table 1). As for pretreatment, reagents including water-borne surfactant and potassium hydroxide (KOH) were used in this study to prepare the specimens before colour protection treatment [14] and [15]. All reagents were purchased from Acros Organics Co. (Geel, Belgium). Bamboo culms and leaves were treated with 0.25% protectors in a 100 °C water bath for 2 h to evaluate the effectiveness of green-colour protection by these preservatives. After treatment, all specimens were dried at 60 °C for 12 h before measurement of surface colour and other properties.