TPH degradation study using isolated fungal strains was
determined by measuring the residual amount of crude oil
present in culture after incubated. Experiments were
carried out in 100 ml shaking flasks containing 50 ml
optimized nutrient media in an incubator shaker at 120
rpm/min shaking speed at optimized temperature and pH
for 30 days. Petroleum oil sludge was added so that the
initial amount of oil in the flask was set at 2.0 %.