The ruminal fluid pH from cows offered the DGM diet was lower (P < 0.01) than the pH in ruminal fluid from cows offered the CON diet Dietary treatment did not influence (P > 0.05) the concentrations of NH3 or total VFA in ruminal fluid However, the ruminal fluid from cows offered the DGM diet contained a lower (P < 0.01) molar percentage of
acetic acid and a greater molar percentage of caproic acid than the ruminal fluid from cows offered the CON diet
No dietary effect was detected on the total number of ciliated protozoa or the numbers of Entodinia spp., and Dasytricha spp. in ruminal fluid. However, the numbers of Isotricha spp. were less (P < 0.05) in the ruminal fluid of cows offered the EGM diet compared with the CON diet. Dietary treatment had no effect (P > 0.05) on the concentrations of H2, CH4, and CO2 in the rumen headspace
than the pH in ruminal fluid from cows offered the CON diet