Venous blood was collected in the heparinized tubes from both normal and occupational sprayers as coded samples from both the control and sprayers. Samples were transported to the analytical toxicology laboratory in ice-cold condition immediately after the collection. The content was centrifuged at 1000g for 10 min for
RBC separation. The buffy coat was removed and the remaining RBCs were drawn from the bottom and then the packed RBCs were washed three times with cold
phosphate-buffered saline (pH 7.4). After the final wash, the RBCs were lysed by hypotonic shock and different dilutions were used as hemolysate.The analysis of different biochemical indices were carried out in the hemolysate on the same day.